Product Description
A HPLC column washing pump is a specific pump utilized in Elite Execution Fluid Chromatography (HPLC) frameworks to convey solvents or cleaning answers for the chromatography column. It is utilized for cleaning and setting up the column for investigation by eliminating buildups from past runs.
Features ofHPLC Column Washing Pump:
- Plug and Play Modular pump.
- Automatic compressibility compensation.
- High Precision and accurate flow rate.
- 5 column washing station and standards.
- Upgradable to low pressure Binary & Quaternary gradient.
- Upgradable to 5 columns station with oven facility.
- Continuous & timed operation.
- Cut-offs pressure setting.
FAQs ofHPLC Column Washing Pump:
Q: For what reason is column washing significant in HPLC?
A: Column washing is fundamental to keep up with the exhibition and life span of HPLC columns. Over the long haul, test deposits, pollutants, and cradle salts can amass on the column, influencing partition proficiency and pinnacle goal. Standard column washing assists with eliminating these contaminations and reestablish column execution.
Q: How does a HPLC column washing pump work?
A: A HPLC column washing pump works in basically the same manner to different pumps in a HPLC framework. It conveys the picked washing dissolvable or arrangement through the column to flush out undesirable buildups. The pumps stream rate and tension control assist with guaranteeing powerful cleaning without harming the column.
Q: What kinds of solvents are utilized for column washing?
A: The decision of washing dissolvable relies upon the idea of the examples run on the column. Regularly utilized solvents incorporate water, natural solvents like methanol or acetonitrile, and combinations of these solvents. The dissolvable ought to be viable with the column material and the analytes being isolated.
Q: How frequently would it be a good idea for me to perform column washing?
A: The recurrence of column washing relies upon elements, for example, the sorts of tests run, the columns ability, and the quantity of runs. As an overall rule, customary column support through washing is suggested after a specific number of runs or when you notice a decrease in partition execution.
Q: Might I at any point involve a similar pump for both example examination and column washing?
A: Indeed, in most HPLC frameworks, a similar pump can be utilized for both example examination and column washing. In any case, its critical to guarantee careful cleaning of the pump and tubing prior to changing from an example to a washing dissolvable to forestall defilement.
Q: Are there explicit directions for utilizing a HPLC column washing pump?
A: The activity of a HPLC column washing pump is like different pumps in the framework. You would set the proper stream rate and tension boundaries, interface the pump to the column delta, and run the picked washing dissolvable through the column for the necessary span.
Digital Flow Rate Control for PrecisionEquipped with an easy-to-use digital control panel, the HPLC Column Washing Pump allows users to adjust the flow rate between 1 to 5 ml/min with accuracy of 2%. This feature ensures optimal cleaning efficiency for any type of HPLC column, minimising waste and enhancing usability for laboratory personnel.
Robust Design and Chemical ResistanceConstructed from high-grade stainless steel and ABS, and fitted with PTFE tubing, the pump offers outstanding longevity and resistance to aggressive cleaning solvents. Its compact dimensions (410 x 230 x 100 mm) and weight (approx. 3.5 kg) make it portable and convenient for different lab environments.
Built-in Safety and Quiet OperationWith built-in overload and leak protection, the pump offers enhanced safety during continuous operation. Emitting less than 55 dB, it operates quietlya crucial benefit in busy laboratory settings. The prime/flush function streamlines column maintenance, improving workflow efficiency.
FAQs of HPLC Column Washing Pump:
Q: How does the digital flow rate adjustability benefit laboratory workflows?
A: The digital flow rate adjustability offers precise control from 1 to 5 ml/min, allowing users to tailor the cleaning process according to specific column requirements. This flexibility enhances cleaning efficiency and conserves cleaning solutions.
Q: What safety features are included to protect users and equipment during column washing?
A: The pump is equipped with overload and leak protection systems, ensuring safe operation even during prolonged use. These features help prevent equipment damage and maintain a safe laboratory environment.
Q: When should the HPLC Column Washing Pump be used in typical laboratory processes?
A: The pump should be used before and after HPLC analyses to thoroughly clean and flush columns, helping to maintain column integrity and reproducibility of analytical results. Regular use can extend column lifespan and improve data reliability.
Q: What solvents and cleaning agents can be used with this pump?
A: Thanks to its chemical-resistant PTFE tubing and stainless steel components, the pump is compatible with a wide range of solvents commonly used in laboratory column cleaning, including those that are aggressive or corrosive.
Q: Where can this HPLC column washing pump be installed or used?
A: The pumps compact and lightweight design allows it to be used in various laboratory workspaces, including academic, research, or industrial labs in India and elsewhere. Its portable form is suitable for bench-top operation.
Q: What are the main benefits of using this column washing pump over manual cleaning?
A: Using this pump provides consistent flow rates, low pulsation, and hands-free operation, reducing manual labor and potential for error. Automated cleaning ensures thorough column maintenance and helps achieve reproducible results in analyses.